Sample information
Picture	Photos by: Angel J
Location
Collection date	11/22/2023
Captive / Cultivated?	Wild-caught
Group	John Overton High School
Observations	it turned into solid quickly
Putative identification	Arthropoda
Methods
Extraction kit	DNeasy (Qiagen)
DNA extraction location	Partial abdomen
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	TAE
DNA stain	SYBR Safe
Gel images
Protocol notes	We had to put on gloves and google like masks
Results
Wolbachia presence	No
Confidence level	Medium
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Arthropoda was found to be negative for Wolbachia.