Fly

November 29, 2023

Sample information
Picture
Photos by: Angel J
Location
Collection date 11/22/2023
Captive / Cultivated? Wild-caught
Group John Overton High School
Observations

it turned into solid quickly
Putative identification Arthropoda

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

We had to put on gloves and google like masks

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Arthropoda was found to be negative for Wolbachia.
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