Sample information |
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| Picture |
|
|---|---|
| Location | |
| Collection date | 09/22/2020 |
| Captive / Cultivated? | Wild-caught |
| Group | Acton-Boxborough Regional High School |
| Observations | This Splendid Earth Boring Beetle was caught in the woods near our school. The cup used to catch the beetle was embedded in the ground and surrounded by lots of leaves which fell from the trees. It’s unclear exactly when the beetle was caught, but the week or so the trap was set out was during autumn. |
| Putative identification | Arthropoda Hexapoda Insecta Coleoptera Geotrupidae Geotrupes Geotrupes splendidus |
Methods |
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| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Partial abdomen |
| Single or Duplex PCR | |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
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| Wolbachia presence | No |
| Confidence level | |
| Explanation of confidence level | |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Geotrupes splendidus was found to be negative for Wolbachia. |
Water Boatman