Splendid Earth Boring Beetle Period 1 Group 6 Gold

September 22, 2020

Sample information
Picture
Location
Collection date 09/22/2020
Captive / Cultivated? Wild-caught
Group Acton-Boxborough Regional High School
Observations

This Splendid Earth Boring Beetle was caught in the woods near our school. The cup used to catch the beetle was embedded in the ground and surrounded by lots of leaves which fell from the trees. It’s unclear exactly when the beetle was caught, but the week or so the trap was set out was during autumn.
Putative identification Arthropoda Hexapoda Insecta Coleoptera Geotrupidae Geotrupes Geotrupes splendidus

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Partial abdomen
Single or Duplex PCR
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Geotrupes splendidus was found to be negative for Wolbachia.
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