Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 01/23/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | |
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera |
Methods |
|
| Extraction kit | |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used. |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | All controls worked as expected, and the DNA specimen had a A60/A80 ratio over 1.7. The experiment went as planned and is supported by information gathered from reliable, peer-reviewed sources. I am confident the Hymenoptera does not have Wolbachia. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Hymenoptera was found to be negative for Wolbachia. |
Water Boatman