Melanoplus sp.

April 16, 2024

Sample information
Picture
Photos by: Janna Mahmoud
Location
Collection date 09/10/2023
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations
Putative identification Arthropoda Hexapoda Insecta Orthoptera Acrididae Melanoplus

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer Other
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level Low
Explanation of confidence level

Although I received positive Wolbachia results, I am not completely confident due to the low-quality levels when it was sequenced. In addition, I did not receive any Wolbachia blast hits when submitting the fasta to the database.
Wolbachia 16S sequence Download FASTA    Download AB1
TTATCCCTTTCCATTGAGTGGGAATGTCCTCCACCCATTACAGTGGCATGTTGATTTATTTTACTAGGGGATTTACTTTTTATACTCTAGTGCGGAGAAAAACAAAATGAAAGGTCTTTTAAAGGTTAGCTTATGGTCGCGCATTTTTTGCCCATTTGGGACCACATTGGTTCACGGGGGTGGCCCATTCCATAAAGGCCGTAAAAAGGGGCATCATCCCCACCTTCTTCCAGCTTATCCCTGGGGGTTTCCTTAATGTACTCCAATTACCTGAGGCGAACTAATGAAGAGAGTTGCGCTCGGTGCGGGAGTTGTCCGGTTTCTCTCGACACCCGCTGACTTCAGCCATGCAAAACTCGTGGGAAAAACGGCCGAACGAATCGTATCTTTTAAATTTCTTTTTTTTTGGGGTTTGGCAAATCTGGGA
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
GCCTCCTCCGGGGTAAGAGATATTTAAATTTCGATCAGTTAATAATATTGTAATAGCTCCTGCTAATACAGGAAGTGATA ATAATAAAAGAGTGCTGTAATAGCTACTGATCAAACAAATAATGGTGTTTGGTCTAGGTTATTCTTTCTGATCGTATATT AATTGCTGTTGTAATAAAATTGACTGCCCCTAGAATTGATGAAACACCAGCTAAGTGAAGAGAGAAAATATAAATCAACT GACGCTCCACCATGTGCAATTGCTCCAGCGAGTGGGGGGTAAACTGTTCATCCTGTACCAGCTCCATTATCAACCATAGA TGAGGTAAGTAAAAGGGTTAGTGATGGTGGTAAAAGTCAAAAACTTATATTATTTATTCGTGGAAAAGCTATATCTGGTG CTCCAATTATTAATGGTACAAGTCAATTACCAAATCCTCCAATTATAATAGGTATTACTATAAAGAAAATTATTACAAAT GCGTGGGCTGTAATAATTACATTATAGATTTGGTCATCTCCAATTAGAGATCCTGGTTGTCCTAGTTCTGCTCGAATAAT TATTCTTATAGAAGTTCCTACTATTCCAGCTCATGCTCCAAATATAAAGTATAAGGTACCAATATCTTTATGATTTGTTG ACCAAGAAAAAAAA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Melanoplus was found to be postive for Wolbachia.
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