Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 02/25/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | College of Eastern Idaho |
| Observations | |
| Putative identification | Arthropoda |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | |
| Buffer | TAE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
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| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
AGGTACTGCTATAAGAGTATTAATTCGTATTGAATTAGGACAACCTGGAAATTTACTTGGGGATGATCAATTATATAATGTTATTGTAACTGCTCACGCTTTTGTTATAATTTTTTTTATAGTTATACCTATTTTAATTGGANGTTTTGGAAATTGATTAGTTCCTTTAATATTAGGTGCGCCTGATATAGCATTTCCTCGTATAAATAATTTGAAATTTTGATTATTACCTCCTTCTTTATTATTATTATTTATTTCTAAAATAGATAAAATAGGAGTGGGTGCGGGTTGAACTATTTATCCCCCTCTTGCTTCTTTACATGGGCATTCTGGGAGATCANGGGATTTTGCTATTTTTTCTTTACTTTTGGGAAGAGCTTCTTCTATTATGGGAGCTATTAATTTTATTTCTACTATTATTAATATACGAGGATATGGAATGACTATANAAAAAGTTCCTTTGTTCGTATGATCTGNTTTGATTACCGNTGTNTTATTATTATTATCTTTACNTGTGNNAGCAGGTGCTATTACTATACTTTTAACA
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Arthropoda was found to be negative for Wolbachia. |
Thrip – Phlaeothripidae Leptothrips sp. 21
MACALF24-SB Dermaptera
Thrip – Phlaeothripidae Leptothrips sp. 19