Oniscus asellus, Onisciadae, MM1

May 16, 2024

Sample information
Picture
Photos by: Maduzaa M.
Location
Collection date 03/14/2024
Captive / Cultivated? Wild-caught
Group KantiWil
Observations

Found under dead tree trunk under small group (2-3 pieces)
Putative identification Arthropoda Crustacea Malacostraca Isopoda Oniscidae Oniscus Oniscus asellus

Methods
Extraction kit Instagene Matrix
DNA extraction location Rear half
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

 

 

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

The result proves that the species “Oniscus asellus” has a Wolbachia sequence. Confidence in the result is high as the marker sequence was correct.
Wolbachia 16S sequence Download FASTA    Download AB1
TGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGATAATGCTGGGGACTTTAAGGAAACTGCTAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGCACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCATGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
STGTAGGTACCTCGCTCAGGATGATTATTCGAACAGAGCTAGGTTCCCCTGGTAGATTAATTGGGGACGACCAGATTTACAATGTTATTGTTACAGCACATGCTTTTGTTATAATTTTCTTTATAGTAATACCTATTATAATTGGAGGTTTTGGTAATTGATTAGTTCCGTTAATATTAGGGGCTCCTGACATAGCATTCCCGCGTATAAATAATATAAGATTTTGGCTTCTCCCACCTTCTTTAGTTCTTCTCCTTAGAAGAGGGTTGATTGAAAGGGGAGTAGGGACTGGCTGAACTGTTTACCCTCCGTTGGCTGGCGGAATTGCTCACAGAGGGGCTTCAGTAGATTTAGGAATTTTTTCTCTTCATCTTGCGGGGGCTTCTTCAATTTTAGGTGCAGTAAATTTTATTACTACTGTAGCTAATATACGTTCTCCTGGTATAAAAATAGATCGGGTGCCTTTATTTGTATGGTCTGTTTTAATTACAGCTGTTTTATTATTACTTTCGTTACCTGTTTTAGCAGGCGCTATTACTATGTTGCTAACTGACCGTAATTTAAATACATCTTTTTTTGATCCTAGCGGGGGAGGAGACCCTATTTTATACCAGCATTTATTTTGATTTTTTGGTCACTTAGAAGTTTAAA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Oniscus asellus was found to be postive for Wolbachia.
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