Rhagium inquisitor MS1

May 16, 2024

Sample information
Picture
Photos by: Magdalena S.
Location
Collection date 03/14/2024
Captive / Cultivated? Wild-caught
Group KantiWil
Observations

caught under bark of a dead tree; dark; wet
Putative identification Arthropoda Hexapoda Insecta Coleoptera Cerambycidae Rhagium Rhagium inquisitor

Methods
Extraction kit Instagene Matrix
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I am confident as there is a line at the cytochrome oxidase but no at the Wolbachia 16S rDNA.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
TGAYCAAATCWATAATGTTATTGTTACTGCCCATGCTTTTATTATAATTTTTTTCATAGTTATACCAATTATAATTGGAGGATTTGGAAACTGATTAGTTCCCCTAATATTGGGGGCTCCCGATATAGCTTTTCCTCGAATAAATAATATAAGATTTTGACTTTTACCTCCTTCTTTAACTTTATTAATCATAAGAAGAGTGGTAGAAAATGGGGCTGGTACCGGATGAACCGTCTATCCCCCTCTTTCTTCAAATATTGCTCATAGAGGATCTTCAGTTGATTTAGCAATTTTTAGGTTACATTTAGCAGGTATCTCTTCAATTCTTGGTGCAGTAGATTTTATCACTACAGTAATTAATATACGACCAATTGGAATAACTCCTGATCGTGTTCCTTTATTTGTTTGGGCAGTAGTAATTACTACAATTCTTCTTCTTCTTTCATTACCAGTTTTAGCTGGAGCAATTACTATACTTTTAACAGATCGAAATTTAAATACTTCATTTTTTGATCCTGCAGGAGGTGGTGATCCTATTCTTTATCAACATTTATTTTGATTTTTTGGTCACTA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Rhagium inquisitor was found to be negative for Wolbachia.
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