Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 03/13/2024 |
| Captive / Cultivated? | Captive / Cultivated |
| Group | College of Eastern Idaho |
| Observations | J1 through J4 tested for Wolbachia 16s gene, J1- pos. control Drosophila , J2-neg. control Drosophila, J3-Ant, J4-Mealworm, J5 through J8 tested for Co1 arthropod gene, J5-Drosophila pos. control for Wolbachia, J6-Drosophila neg. control for Wolbachia, J7-ant, J8-Mealworm, arrows indicate positive test, J8-only pos. that was not a control 13MAR2024 |
| Putative identification | Arthropoda Hexapoda Insecta Coleoptera Tenebrionidae Tenebrio Tenebrio molitor |
Methods |
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| Extraction kit | Monarch DNA extraction (NEB) |
| DNA extraction location | |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | LB |
| DNA stain | GelGreen |
| Gel images |
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| Protocol notes | |
Results |
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| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
jaime_samp2_CO1_1C_Tenebrio_molitor ATCCGGATAGTTGGAACCTCTCTAAGACTATTAATCCGTGCAGAATTAGGAAACCCCGGCTCTTTAATTGGAGACGACCAAATCTACAACGTAATTGTTACAGCACATGCTTTTATCATAATTTTTTTCATAGTAATACCAATCATAATTGGAGGATTCGGGAATTGATTAGTACCTCTAATACTAGGTGCTCCTGACATAGCATTCCCGCGAATAAACAACATAAGATTCTGATTGCTACCCCCTTCACTAAGACTATTACTAATAAGAAGAATTGTAGAAAACGGGGCAGGAACCGGTTGGACAGTTTATCCGCCTTTATCCTCTAATATCGCCCATGGGGGAGCATCTGTCGATTTAGCAATTTTCAGGTTACATCTAGCAGGAATTTCATCAATCCTGGGGGCCGTAAATTTTATTACAACAGTAATCAACATACGACCACAGGGTATAACATTCGACCGAATACCGTTATTCGTATGAGCAGTAGTAATTACCGCCGTACTATTATTATTATCATTACCAGTATTAGCAGGAGCCATTACCATATTACTTACAGATCGAAACATTAATACATCCTTCTTTGACCCAGCGGGGGGAGGAGACCCAATCCTATACCAACACCTATTCTGATTTTTTGGTCAC
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Tenebrio molitor was found to be negative for Wolbachia. |
Anyphaena accentuata NP1
Cylindroiulus caeruleocinctus NP2
Lithobius forficatus SM1
Oniscus asellus GC1
Oniscus Asellus (LS1)