Tenebrio molitor

April 25, 2024

Sample information
Picture
Photos by: Jaime R.
Location
Collection date 03/13/2024
Captive / Cultivated? Captive / Cultivated
Group College of Eastern Idaho
Observations

J1 through J4 tested for Wolbachia 16s gene, J1- pos. control Drosophila , J2-neg. control Drosophila, J3-Ant, J4-Mealworm, J5 through J8 tested for Co1 arthropod gene, J5-Drosophila pos. control for Wolbachia, J6-Drosophila neg. control for Wolbachia, J7-ant, J8-Mealworm, arrows indicate positive test, J8-only pos. that was not a control

13MAR2024
Putative identification Arthropoda Hexapoda Insecta Coleoptera Tenebrionidae Tenebrio Tenebrio molitor

Methods
Extraction kit Monarch DNA extraction (NEB)
DNA extraction location
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer LB
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
jaime_samp2_CO1_1C_Tenebrio_molitor ATCCGGATAGTTGGAACCTCTCTAAGACTATTAATCCGTGCAGAATTAGGAAACCCCGGCTCTTTAATTGGAGACGACCAAATCTACAACGTAATTGTTACAGCACATGCTTTTATCATAATTTTTTTCATAGTAATACCAATCATAATTGGAGGATTCGGGAATTGATTAGTACCTCTAATACTAGGTGCTCCTGACATAGCATTCCCGCGAATAAACAACATAAGATTCTGATTGCTACCCCCTTCACTAAGACTATTACTAATAAGAAGAATTGTAGAAAACGGGGCAGGAACCGGTTGGACAGTTTATCCGCCTTTATCCTCTAATATCGCCCATGGGGGAGCATCTGTCGATTTAGCAATTTTCAGGTTACATCTAGCAGGAATTTCATCAATCCTGGGGGCCGTAAATTTTATTACAACAGTAATCAACATACGACCACAGGGTATAACATTCGACCGAATACCGTTATTCGTATGAGCAGTAGTAATTACCGCCGTACTATTATTATTATCATTACCAGTATTAGCAGGAGCCATTACCATATTACTTACAGATCGAAACATTAATACATCCTTCTTTGACCCAGCGGGGGGAGGAGACCCAATCCTATACCAACACCTATTCTGATTTTTTGGTCAC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Tenebrio molitor was found to be negative for Wolbachia.
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