Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 08/25/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Kantonsschule Zuercher Unterland |
| Observations | |
| Putative identification | Arthropoda Hexapoda Insecta Coleoptera |
Methods |
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| Extraction kit | Instagene Matrix |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | Midori 1:20'000 |
| Gel images |
|
| Protocol notes | despite the faint band we received decent sequences |
Results |
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| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | we’ve run the analysis twice; BLAST of the consensus sequence resultet in 12 hits with > 98.6% identity for Protapian apricans |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
Download AB1
TATTGAATTAGGTAACCCTGGATCATTAATTGGTAATGATCAAATTTATAATGTTATTGTCACAGCTCATGCATTTATTATAATTTTTTTTATAGTTATACCTGTTATAATTGGAGGATTTGGTAATTGATTAATTCCTTTAATATTAGGAGCACCTGATATGGCATTTCCTCGAATAAATAATATAAGATTTTGACTTTTACCTCCTTCTCTAACTCTTTTATTAATAAGAAGAATTATTGAAAAAGGCGCAGGAACCGGATGAACTGTTTACCCTCCTTTAGCTGCTAATATTGCTCACAGAGGAGCTTCTGTTGATTTAGCAATTTTTAGTCTCCATTTAGCTGGAATCTCATCAATTTTAGGTGCTGTAAATTTTATTTCTACTATTATAAATATACNACCTACTGGAATATCTCTTGATCAACTATCACTTTTCACTTGAGCTGTAAAAATTACAGCTATTCTACTTTTATTATCTTNACCTGTTTTANCAGGGGC
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Coleoptera was found to be negative for Wolbachia. |
Water Boatman