Protapion apricans

May 24, 2024

Sample information
Picture
Photos by: Alena B. and Jorina K.
Location
Collection date 08/25/2023
Captive / Cultivated? Wild-caught
Group Kantonsschule Zuercher Unterland
Observations
Putative identification Arthropoda Hexapoda Insecta Coleoptera

Methods
Extraction kit Instagene Matrix
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain Midori 1:20'000
Gel images
Protocol notes

despite the faint band we received decent sequences

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

we’ve run the analysis twice;
within the same PCR run other groups found Wolbachia within ther samples

BLAST of the consensus sequence resultet in 12 hits with > 98.6% identity for Protapian apricans
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
TATTGAATTAGGTAACCCTGGATCATTAATTGGTAATGATCAAATTTATAATGTTATTGTCACAGCTCATGCATTTATTATAATTTTTTTTATAGTTATACCTGTTATAATTGGAGGATTTGGTAATTGATTAATTCCTTTAATATTAGGAGCACCTGATATGGCATTTCCTCGAATAAATAATATAAGATTTTGACTTTTACCTCCTTCTCTAACTCTTTTATTAATAAGAAGAATTATTGAAAAAGGCGCAGGAACCGGATGAACTGTTTACCCTCCTTTAGCTGCTAATATTGCTCACAGAGGAGCTTCTGTTGATTTAGCAATTTTTAGTCTCCATTTAGCTGGAATCTCATCAATTTTAGGTGCTGTAAATTTTATTTCTACTATTATAAATATACNACCTACTGGAATATCTCTTGATCAACTATCACTTTTCACTTGAGCTGTAAAAATTACAGCTATTCTACTTTTATTATCTTNACCTGTTTTANCAGGGGC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Coleoptera was found to be negative for Wolbachia.
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