GS17 Roly1

May 25, 2024

Sample information
Picture
Photos by: Joshua YK, Benjamin T, Briant F, Edward H, Bryan F, Joseph L.
Location
Collection date 05/15/2024
Captive / Cultivated? Wild-caught
Group California Academy of Mathematics and Science
Observations

Rolypoly caught under a broken piece of wood logs just outside of CAMS parking lot.
Putative identification Arthropoda Crustacea Malacostraca Isopoda Armadillidae Armadillidium Armadillidium vulgare

Methods
Extraction kit tweezers
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Fast Blast
Gel images
Protocol notes

130 Volts, 60 Minutes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Armadillidium vulgare was found to be negative for Wolbachia.
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