Camponotus chromaiodes

May 28, 2024

Sample information
Picture
Photos by: Mia C.
Location
Collection date 05/28/2024
Captive / Cultivated? Wild-caught
Group Pingry School
Observations

Black body, red abdomen and legs
Putative identification Arthropoda Insecta Hymenoptera Formicidae Camponotus Camponotus chromaiodes

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Protocol ran smoothly. The second ladder (1st after ladder) is the C. chromaiodes.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

The controls worked. There is a band along with the positive Wolbachia control and DNA control.
Wolbachia 16S sequence Download FASTA    Download AB1
GTGTTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCACGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGTTTCA
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
CTTTGCTATTTGATCGNNANNGTTGGCTCCTCTATAAGAATAATCATTCGACTAGAGTTGGGATCTCCTGATTCACTAATTCTTAATGATCAAACTTTCAATACCATCGTTACAAGTCATGCTTTTATTATAATTTTTTTTATAGTTATACCTTTTATAATTGGGGGATTTGGTAATTTTTTAATTCCCCTTATACTAGGATCTCCTGATATAGCTTACCCCCGTTTAAATAACATAAGATTTTGATTACTTCCCCCATCGATCTCCTTATTAATCCTAAGAAATTTTATTAATGAAGGATCTGGAACTGGTTGGACTGTCTACCCCCCTCTATCATCAAATACCTTCCATAGTGGCCCCTCTATTGACCTGACTATCTTTTCTCTCCATATTGCTGGTATATCCTCAATTATAGG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Camponotus chromaiodes was found to be postive for Wolbachia.
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