Anoecia fulviabdominalis_RS2

September 16, 2024

Sample information
Picture
Photos by: Rea S.
Location
Collection date 08/28/2024
Captive / Cultivated? Wild-caught
Group KantiWil
Observations

Approx. 1.50 metres above the forest floor in an area where the sun’s rays reach well.
Putative identification Arthropoda Hexapoda Insecta Hemiptera

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

The gel image shows that the sample is Wolbachia positive.
Wolbachia 16S sequence Download FASTA    Download AB1
CTTGKAACCCATTAATTAATAATGATCAAATTTATAATTCTCTTGTAACTAGCCATGCCWWTATTATAATTTTTTTTATAGTTATACCATTTATAATTGGGGGCTTTGGTAATTTTTTAATCCCTTTAATATTAGGGTCACCTGATATAGCATACCCMCGAATAAATAACATAAGATTTTGATTACTTCCCCCTTCAATTATACTATTAATATTAAGAAATTTTTTAAGGAATGGAGKAGGKRCRGGATGAACCATTTACCCCCCTCYAGCTTCTAATATWTTCCWTAGAGGACCTTCAATTGATATATSARKCKTTTCTCTTCATATCGCTGGAATATCAKCT
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
TTAGGATCTTGTAACCCATTAATTAATAATGATCAAATTTATAATTCTCTTGTAACTAGCCATGCCTTTATTATAATTTTTTTTATAGTTATACCATTTATAATTGGGGGCTTTGGTAATTTTTTAATCCCTTTAATATTAGGGTCACCTGATATAGCATACCCACGAATAAATAACATAAGATTTTGATTACTTCCCCCTTCAATTATACTATTAATATTAAGAAATTTTTTAAGGAATGGAGTAGGTACAGGATGAACCATTTACCCCCCTCTAGCTTCTAATATTTTCCATAGAGG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Hemiptera was found to be postive for Wolbachia.
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