Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/03/2024 |
| Captive / Cultivated? | Captive / Cultivated |
| Group | Galileo Academy of Science and Technology |
| Observations | |
| Putative identification | Arthropoda Hexapoda Insecta Diptera Culicidae Aedes |
Methods |
|
| Extraction kit | BABEC |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TAE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | Taq polymerase used Annealing temperatures for each PCR reaction was 55 degrees celsius. 6th well starting from left to right a |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Aedes was found to be postive for Wolbachia. |
Water Boatman