Sample information
Picture	Photos by: Bindy G.
Location
Collection date	09/03/2024
Captive / Cultivated?	Captive / Cultivated
Group	Galileo Academy of Science and Technology
Observations	Raised in a mosquito breeder, female mosquito with no furry antennas
Putative identification	Arthropoda Hexapoda Insecta Diptera Culicidae Culex Culex pipiens
Methods
Extraction kit	BABEC
DNA extraction location	Whole arthropod
Single or Duplex PCR	Duplex Reaction
Gel electrophoresis system	MiniOne
Buffer	TAE
DNA stain	GelGreen
Gel images
Protocol notes	BABEC’s protocol
Results
Wolbachia presence	No
Confidence level	Medium
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Culex pipiens was found to be negative for Wolbachia.