Theo-Parasteatoda tepidariorum voucher

Sample information

Picture
Photos by: Theo H.
Location
Collection date 09/04/2024
Captive / Cultivated? Wild-caught
Group St. Albans School
Observations

Caught in a house, at room temperature. Arthropod was found in web in the corner of my house.

Putative identification Arthropoda Chelicerata Arachnida Araneae

Methods

Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results

Wolbachia presence No
Confidence level High
Explanation of confidence level

Our controls worked and no unexpected results occurred.

Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
GTAGATTTATGGGTGATGATCAATTATATAATGTAATTGTAACGGCTCATGCTTTTGTAATAATTTTTTTTATAGTAATACCAATTTTAATTGGAGGGTTTGGAAATTGATTAGTTCCATTAATATTAGGGGCACCTGACATAGCATTTCCTCGAATAAATAATTTGAGATTTTGATTATTACCCCCTTCTTTATTATTATTATTTATTTCGTCTATAGTAGAAATAGGAGTAGGAGCTGGGTGAACTGTATATCCACCTCTATCATCTTTAGATGGTCACGGTGGTAGATCAGTAGATTTTGCAATTTTTTCTTTGCATTTAGCTGGGGCTTCTTCTATTATAGGTGCGGTAAATTTTATTTCTACTGTACTCAATATGCGATCTTATGGGATGAGAATAGAAAAAGTTCCTTTATTTGTATGATCAGTTCTAATTACTGCTGTGCTATTATTATTATCATTACCAGTATTAGCAGGTGCTATTACTATATTATTAACTGATCGAAATTTTAATACTTCATTTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Araneae was found to be negative for Wolbachia.
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