Sample information |
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Picture |
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Location | |
Collection date | 08/20/2024 |
Captive / Cultivated? | Wild-caught |
Group | Kantonsschule Zuercher Unterland |
Observations | The bug was found near a flowerbed on a hot stone with in it‘s colony. |
Putative identification | Arthropoda Hexapoda Insecta Hemiptera Pyrrhocoridae Pyrrhocoris |
Methods |
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Extraction kit | Instagene Matrix |
DNA extraction location | Abdomen |
Single or Duplex PCR | Single Reaction |
Gel electrophoresis system | MiniOne |
Buffer | |
DNA stain | Midori 1:20'000 |
Gel images |
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Protocol notes | This bug didn‘t show any results on both PCRs. This might be due to an error in the DNA extraction or the PCR process. There is a possibility that some chemicals or bad conditions lead to the DNA degrading. |
Results |
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Wolbachia presence | Unknown |
Confidence level | Low |
Explanation of confidence level | There were no results in both PCRs. The second PCR was even done by the teacher. |
Wolbachia 16S sequence | |
Arthropod COI sequence |
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Summary |