Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 08/20/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Kantonsschule Zuercher Unterland |
| Observations | The bug was found near a flowerbed on a hot stone with in it‘s colony. |
| Putative identification | Arthropoda Insecta Hemiptera Pyrrhocoridae Pyrrhocoris |
Methods |
|
| Extraction kit | Instagene Matrix |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | |
| DNA stain | Midori 1:20'000 |
| Gel images |
|
| Protocol notes | This bug didn‘t show any results on both PCRs. This might be due to an error in the DNA extraction or the PCR process. There is a possibility that some chemicals or bad conditions lead to the DNA degrading. |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Low |
| Explanation of confidence level | There were no results in both PCRs. The second PCR was even done by the teacher. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid