Aedes japonicus japonicus

October 22, 2024

Sample information
Picture
Photos by: Luna. S, Tharanya. S
Location
Collection date 08/21/2024
Captive / Cultivated? Wild-caught
Group Kantonsschule Zuercher Unterland
Observations

Captured: Glattfelden forest
Condition: Dead when found (did not freeze to death)
Putative identification Arthropoda

Methods
Extraction kit Instagene Matrix
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer
DNA stain Midori 1:20'000
Gel images
Protocol notes

In both PCR processes strong results were observed.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

In both PCR processes there was a clear result. One was made by the students and the other one by our teacher. In both cases the lines are perfectly visible.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
Summary The Arthropoda was found to be negative for Wolbachia.
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