Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 08/20/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Kantonsschule Zuercher Unterland |
| Observations | in garden on leaf of a raspberry bush |
| Putative identification | Arthropoda Hexapoda Insecta Hemiptera Pentatomidae Nezara Nezara viridula |
Methods |
|
| Extraction kit | Instagene Matrix |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | |
| DNA stain | |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | Low |
| Explanation of confidence level | our use of the pipets was a bit too quick and imprecise we might have used too much of the MASTER-Mix |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Nezara viridula was found to be postive for Wolbachia. |
Water Boatman