Sample information |
|
| Picture |
|
|---|---|
| Location | No map location data available. |
| Collection date | 08/20/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Kantonsschule Zuercher Unterland |
| Observations | Caught in my garden |
| Putative identification | Arthropoda |
Methods |
|
| Extraction kit | Instagene Matrix |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | |
| DNA stain | Midori 1:20'000 |
| Gel images |
|
| Protocol notes | Something went wrong during the DNA extraction because there was no sign of a line. |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Low |
| Explanation of confidence level | We are struggling to see our mistake during the whole process of preparing the PCR because every step was made by the same person and in the same way and in other cases there were clear results. Furthermore ovaries with eggs were found here which should have given us clear results. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Water Boatman