Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 09/04/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | St. Albans School |
| Observations | Under a couple of leaves of a bush, on top of a wooden plank, summer, 75°, terrestrial habitat |
| Putative identification | Arthropoda Crustacea Malacostraca Isopoda Armadillidae Armadillidium |
Methods |
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| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
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| Protocol notes | |
Results |
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| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | All of our controls seemed to appear just fine on the gel and we did not have any unexpected difficulties with our electrophoresis process. There is great variety in our gel, and my DNA simply doesn’t have any indication of Wolbachia. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
GCTTGGGCTGCAATAGTGGGAACAGCAATAAGAGTANATTCGAATTGAGTTAGGTCAAACAGGTAGATTTATGGGTGATGATCAATTATATAATGTAATTGTAACGGCTCATGCTTTTGTAATAATTTTTTTTATAGTAATACCAATTTTAATTGGAGGGTTTGGAAATTGATTAGTTCCATTAATATTAGGGGCACCTGACATAGCATTTCCTCGAATAAATAATTTGAGATTTTGATTATTACCCCCTTCTTTATTATTATTATTTATTTCGTCTATAGTAGAAATAGGAGTAGGAGCTGGGTGAACTGTATATCCACCTCTATCATCTTTAGATGGTCACGGTGGTAGATCAGTAGATTTTGCAATTTTTTCTTTGCATTTAGCTGGGGCTTCTTCTATTATAGGTGCGGTAAATTTTATTTCTACTGTACTCAATATGCGATCTTATGGGATGAGAATAGAAAAAGTTCCTTTATTTGTATGATCAGTTCTAATTACTGCTGTGCTATTATTATTATCATTACCAGTATTAGCAGGTGCTATTACTATATTATTAACTGATCGAAATTTTAATACTTCATTTTTTGATCCTGCAGGAGGAGGTGATCCTATTTTGTTTCAGCATTTATTCTGATTTT
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Armadillidium was found to be negative for Wolbachia. |
Water Boatman