Herz #06

October 24, 2024

Sample information
Picture
Photos by: Lea R., Louisa R.
Location
Collection date 08/25/2024
Captive / Cultivated? Wild-caught
Group Kantonsschule Zuercher Unterland
Observations

captured: in the garden on a pear in Oberweningen

condition: dead when caught, then frozen and preserved in alcohol
Putative identification Arthropoda Hexapoda Insecta Hymenoptera Vespidae

Methods
Extraction kit Instagene Matrix
DNA extraction location
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain Midori 1:20'000
Gel images
Protocol notes

In both PCR processes meaningful/good results could be observed. We refer to the results in lane 8 (picture 1) and lane 3 (picture 2).

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

In both PCR processes meaningful/good results could be observed. We refer to the results in lane 6 (picture 1) and lane 2 (picture 2). In the first process you can see a soft/weak line/mark on the gel. It’s not as strong as in the other two lanes that showed a mark.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Vespidae was found to be negative for Wolbachia.
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