Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 08/27/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | Location of Collection: Georgia Southern’s aquaponics greenhouse. Savannah, Georgia, 31419 Habitat: The edges of long grass, near a water source. Not far from a walking path. Method of Collection: My partner and I collected our sample using a Sweep Net and swept the blades of grass for insects |
| Putative identification | Arthropoda Insecta Hemiptera |
Methods |
|
| Extraction kit | |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used. |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | Due to proper lab techniques, I performed PCR and Gel Electrophoresis as well as I could. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Hemiptera was found to be negative for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid