MATAEF24-BTP (A) – Hemiptera

November 5, 2024

Sample information
Picture
Photos by: Benjamin Padgett
Location
Collection date 08/27/2024
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Location of Collection: Georgia Southern’s aquaponics greenhouse. Savannah, Georgia, 31419

Habitat: The edges of long grass, near a water source. Not far from a walking path.

Method of Collection: My partner and I collected our sample using a Sweep Net and swept the blades of grass for insects
Putative identification Arthropoda Hexapoda Insecta Hemiptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Due to proper lab techniques, I performed PCR and Gel Electrophoresis as well as I could.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hemiptera was found to be negative for Wolbachia.
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