Max’s beetle

Sample information

Picture
Photos by: Max E
Location
Collection date 10/03/2024
Captive / Cultivated? Wild-caught
Group Scarborough High School
Observations
Putative identification Arthropoda Insecta Coleoptera Staphylinidae Ocypus Ocypus olens

Methods

Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Partial abdomen
Single or Duplex PCR
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results

Wolbachia presence No
Confidence level Medium
Explanation of confidence level

Negative for Wolbachia but no DNA ladder

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Ocypus olens was found to be negative for Wolbachia.
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