Small Honey Ant

April 9, 2025

Sample information
Picture
Photos by: Nithil V.
Location
Collection date 11/12/2024
Captive / Cultivated? Wild-caught
Group Walton High School
Observations

This ant was found crawling on a tree. Under the tree there appeared to be a nest covered in leaves where other ants walked out of.
Putative identification Arthropoda Hexapoda Insecta Hymenoptera Formicidae

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Lane 11 contains my sample for this arthropod.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

On our wolbachia gel there was no bar at the 438 mark, indicating that there was no Wolbachia DNA in my sample.
Wolbachia 16S sequence
Arthropod COI sequence Download AB1
NNNNTNNNNNNNGNCNTCTGANCTGGATAATTGGATCTTCNATAAGAATACTTATTCGTTTAGAATTAGGTACTTCAAATTCATTAATTAATAATGATCAAGTTTTTAATTCTATATTTACTAGACATGCTTTTATTATAATTTTTTTTATAGTCATACCTTTTATAATTGGTGGATTTGGAAATTTTTTAGTTCCTTTAATACTAGGATCTCCAGATATAGCCTATCCTCGAATAAATAATATAAGATTTTGACTTTTACCCCCTTCAATCAGTTTACTTTTATTAAGAAATTTCATTAATGACGGAGTGGGAACAGGATGAACTGTTTACCCTCCATTAGCTTCTAATATTTTTCATAATGGTCCTTCAGTTGACCTGACAATTTTTTCTCTACATATTGCAGGAATATCTTCTATTCTAGGAGCTATCAATTTTATTTCAACAATTCTAAATATACGACATAAAAATTTTTCTTCTGATAAAATCCCTTTACTTGTTTGATCTATTTTAATTACAGCAATTTTACTTCTTCTATCTTTACCAGTTCTAGCTGGAGCTATTACTATACTTTTAACTGACCGGAACCTTAATACTTCCTTTTTTGACCCCTCAGGAGGAGGAGACCCTGTTCTTTTATCAACATTTATTCTGATTTTTTGGTCACCCTGAANTTNAAAA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Formicidae was found to be negative for Wolbachia.
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