Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 09/29/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | College of Eastern Idaho |
| Observations | Very small approximately 1 mm in length, dark color. Found in bark of Douglas-fir tree with infestation markings, under bark above the phloem many same insects found.
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| Putative identification | Arthropoda Hexapoda Insecta Thysanoptera Phlaeothripidae Leptothrips |
Methods |
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| Extraction kit | Edwards Buffer |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
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| Protocol notes | |
Results |
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| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | PCR of similar species at same time were positive. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
TTGATCTGGAATTTGTGGATTATTTATAAGAATAATTATTCGTTTAGAATTAATACAACCAGGTAACGTTTTTTTATCTGGTCAAGCTTATAATGTAATTGTAACTAATCATGCTTTTATAATAATTTTTTTTACTATTATACCTATTATAATAGGAGGGTTTGGAAATTGATTAGTTCCTATTATACTAGGTAGACCAGATATATGTTACCCACGTATAAATAATATAAGATTTTGATTATTACCTCCTTCTCTATTTCTTCTCTTATTTAGAATAATTTTAGAAGAAGGTTCAGGAACTGGATGAACGGTTTATCCCCCTTTATCATCTTTAAGATCAGGAATTTCTGTTGATATAACTATTATTTCATTACATATTGCTGGTGTTTCTTCTTTATTAGGATCTATTAATTTTATTTCTACAGTATTTAACATATCTAATTTAAGAACTGAATATTATACTTTATTTGTTTGATCAATTGTAGTTACTTCTTTACTATTATTACTTTCTTTACCTGTATTAGCAGGTGCTTTAACAATATTATTGGTTGATCGAAATTTAAACACTAGTTATTTTGATCCTTCAGGTGGAGGAGATCCTACTTTATATCAACATTTATTTTGATTTTTTGGTC
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Leptothrips was found to be negative for Wolbachia. |
Water Boatman