Thrip – Phlaeothripidae Leptothrips sp. 15

November 15, 2024

Sample information
Picture
Photos by: Heather Ahlstrom
Location
Collection date 09/29/2024
Captive / Cultivated? Wild-caught
Group College of Eastern Idaho
Observations

Very small approximately 1 mm in length, dark color. Found in bark of Douglas-fir tree with infestation markings, under bark above the phloem many same insects found.
Putative identification Arthropoda Hexapoda Insecta Thysanoptera Phlaeothripidae Leptothrips

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR
Gel electrophoresis system Standard electrophoresis system
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

PCR of similar species at same time were positive.
Wolbachia 16S sequence
GCNTGGCTGTCGTCAACTCGTGTCGTGAGATGTTGGGTTAAGTTCCTCAACCAGCGCAACCCTCATCCTTANTTGCCATCAGGTAATGCTGAGCACTTTAAGGAAACTGCCATTGATAAGCTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCTTTATGAAGTGGGCTACACACGTGCTACAATGGTGTCNACAATGGGCTGCAAGGTGCGCAAGCCTAAGCTAATCCCTAAAAGACATCCCATTTCTGATTGTACTCTGCAACTCNAGTGCATGAAATTGGAATCGCTAGTAATCGTGGATCATCATGCCACGGTGAATACNTTCTCGGGTCTTGTANACACTGCCCNNCACACAATGCNAATTTTTTTCACTNNAAGGNTNGTGCTNCNCCTCTATTAGGGGC
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
GATCTGGAATTTGTGGATTATTTATAAGAATAATTATTCGTTTAGAATTAATACAACCAGGTAACGTTTTTTTATCTGGTCAAGCTTATAATGTAATTGTAACTAATCATGCTTTTATAATAATTTTTTTTACTATTATACCTATTATAATAGGAGGGTTTGGAAATTGATTAGTTCCTATTATACTAGGTAGACCAGATATATGTTACCCACGTATAAATAATATAAGATTTTGATTATTACCTCCTTCTCTATTTCTTCTCTTATTTAGAATAATTTTAGAGGAAGGTTCAGGAACTGGATGAACGGTTTATCCCCCTTTATCATCTTTAAGATCAGGAATTTCTGTTGATATAACTATTATTTCATTACATATTGCTGGTGTTTCTTCTTTATTAGGATCTATTAATTTTATTTCTACAGTATTTAACATATCTAATTTAAGAACTGAATATTATACTTTATTTGTTTGATCAATTGTAGTTACTTCTTTACTATTATTACTTTCTTTACCTGTATTAGCAGGTGCTTTAACAATATTATTGGTTGATCGAAATTTAAACACTAGTTATTTTGATCCTTCAGGTGGAGGAGATCCTACTTTATATCAACATTTATTTTGATTTTTTG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Leptothrips was found to be postive for Wolbachia.
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