Flat-backed Millipede

April 4, 2025

Sample information
Picture
Photos by: Navya K.
Location
Collection date 11/12/2024
Captive / Cultivated? Wild-caught
Group Walton High School
Observations

The millipede was found in the soil buried under leave litter and it curled into a spiral upon being collected.
Putative identification Arthropoda Myriapoda Diplopoda Polydesmida Polydesmidae Polydesmus Polydesmus angustus

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

The DNA PCR process was not succesful because the control bands did not correlate.

Results
Wolbachia presence Unknown
Confidence level Low
Explanation of confidence level

My confidence level is low because the control did not match up in the gel.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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