Fruit Fly

Sample information

Picture
Location No map location data available.
Collection date 10/16/2020
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations
Putative identification Hexapoda Insecta Diptera

Methods

Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

DNA extraction very small pellet.

 

Results

Wolbachia presence No
Confidence level Medium
Explanation of confidence level

Wolbachia gel: Wolbachia DNA and control didn’t work

Arthropod gel: Controls didn’t work

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Diptera was found to be negative for Wolbachia.
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