Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 11/12/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Walton High School |
| Observations | -attempt to hide under pieces of soil in collection tube -segmented body, abdomen darker in color with some pattern and spotting -lighter colored, segmented legs |
| Putative identification | Arthropoda Chelicerata Arachnida Araneae Theridiidae Steatoda Steatoda triangulosa |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | The controls for Wolbachia were successful when the gel was run, but there was no visible band. The sample was not sent for sequencing. |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Low |
| Explanation of confidence level | We did not get good results in the arthropod gel, indicating that we messed up somewhere else in the process because the specimen should have been positive for the arthropod gene |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Monochamus Scutellatus MMH10
Eleodes Armata MMH9
Gryllus Veletis MMH8
Trochosa Ruricola MMH7
Apis Mellifera MMH6 L6