Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 10/17/2020 |
| Captive / Cultivated? | Wild-caught |
| Group | Hampton Roads Academy |
| Observations | Visible pellet |
| Putative identification | Arthropoda Hexapoda Insecta Orthoptera |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | Arthropod gel – controls didn’t work Wolbachia gel- Controls and Wolbachia DNA didn’t work
|
Results |
|
| Wolbachia presence | Yes |
| Confidence level | Medium |
| Explanation of confidence level | Issues with controls |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Orthoptera was found to be postive for Wolbachia. |
Extraction and Analysis of Wolbachia from Halyomorpha halys
Tick
Roly-Poly
Idaho Beetle
Grammonota