Sample information |
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Picture |
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Location | |
Collection date | 10/15/2024 |
Captive / Cultivated? | Wild-caught |
Group | Portland State University |
Observations | |
Putative identification | Arthropoda Chelicerata Arachnida Araneae Araneidae Araneus Araneus diadematus |
Methods |
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Extraction kit | DNeasy (Qiagen) blood and tissue kit |
DNA extraction location | Partial abdomen |
Single or Duplex PCR | Single Reaction |
Gel electrophoresis system | Standard electrophoresis system |
Buffer | TAE |
DNA stain | GelRed |
Gel images |
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Protocol notes | |
Results |
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Wolbachia presence | No |
Confidence level | High |
Explanation of confidence level | Gel A, Wells 17-19. Well 17 is positive for arthropod DNA, well 18 is positive for Wolbachia positive control, well 19 is negative for Wolbachia in the spider |
Wolbachia 16S sequence | |
Arthropod COI sequence |
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Summary | The Araneus diadematus was found to be negative for Wolbachia. |