Portland State University – Araneus diadematus

Sample information

Picture
Location
Collection date 10/15/2024
Captive / Cultivated? Wild-caught
Group Portland State University
Observations
Putative identification Arthropoda Chelicerata Arachnida Araneae Araneidae Araneus Araneus diadematus

Methods

Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain GelRed
Gel images
Protocol notes

Results

Wolbachia presence No
Confidence level High
Explanation of confidence level

Gel A, Wells 17-19. Well 17 is positive for arthropod DNA, well 18 is positive for Wolbachia positive control, well 19 is negative for Wolbachia in the spider

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Araneus diadematus was found to be negative for Wolbachia.
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