Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 10/15/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Portland State University |
| Observations | |
| Putative identification | Arthropoda Arachnida Araneae Araneidae Araneus Araneus diadematus |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Partial abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | GelRed |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | Gel A, Wells 17-19. Well 17 is positive for arthropod DNA, well 18 is positive for Wolbachia positive control, well 19 is negative for Wolbachia in the spider |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Araneus diadematus was found to be negative for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid