Portland State University – Aphididae

November 25, 2024

Sample information
Picture
Photos by: Patrick Gresh
Location
Collection date 10/17/2024
Captive / Cultivated? Wild-caught
Group Portland State University
Observations
Putative identification Arthropoda Hexapoda Insecta Hemiptera Aphididae

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain GelRed
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Well 2 is positive for the insect DNA, well 1 is negative for the Wolbachia in the insect. Wells 3 and 4 are the insect and Wolbachia results for a Wolbachia positive fruit fly.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Aphididae was found to be negative for Wolbachia.
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