Sample information |
|
| Picture |
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|---|---|
| Location | |
| Collection date | 09/03/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Benedictine University |
| Observations | IT WAS A DRY WARM DAY. WE COLLECTED BY THE POND NEAR BENEDICTINE UNIVERSITY PARKING GARAGE |
| Putative identification | Arthropoda Insecta Lepidoptera |
Methods |
|
| Extraction kit | Qiagen Blood and Tissue kit |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | Other |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
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| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | WOLBACHIA BAND WAS NOT FOUND ON MY GEL ELECTROPHORESIS. HOWEVER, ACCORDING TO DATA OVER THE INTERNET IT DIDNT STATE THAT MY INSECT DOES HAVE WOLBACHIA. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
>47-A_AG1_Co1R (1).scf (380 bp) AAAACAGGTAAAGATAAGAGTAACAATAAAGCTGTAATTCCTACAGATCAAACAAATAAAGGTATTTGGTCAAATGATAA ATTTTTAATTCGTATATTAATGATTGTTGTAATAAAATTAATAGCTCCTAAAATTGAAGAGATTCCAGCTAAATGAAGAG AAAAAATTGCTAAATCAACAGAAGATCCTTGATGAGCAATATTAGAAGATAAAGGGGGGTAAACTGTTCAACCTGTTCCT GCACCATTTTCTACAATTCTTCTTGAAATTAATAATATTAATGAAGGAGGTAATATTCAAAAACTTATATTATTTATTCG AGGGAAAGCTATATCAGGAGCTCCTAGTATTAAAGGTACTAATCAATTTCCAAATCCTCC
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Lepidoptera was found to be negative for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid