Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/09/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | St. Paul Academy and Summit School |
| Observations | This cicada was found lying on its back on concrete in front of my school. It was 84 degrees out and sunny. it was 6:00 pm when I found it. It was alone and was flapping its wings on the ground. |
| Putative identification | Arthropoda Hexapoda Insecta Hemiptera Cicadidae Magicicada |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Partial abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | |
| Wolbachia 16S sequence |
NA
BLAST at The Wolbachia Project BLAST at NCBI
|
| Arthropod COI sequence |
NA
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Magicicada was found to be negative for Wolbachia. |
Araneae
Woodlouse
Common Water Strider (Aquarius remigis)
Water Strider
Water Strider