spider

April 1, 2025

Sample information
Picture
Location
Collection date 09/21/2024
Captive / Cultivated? Wild-caught
Group St. Paul Academy and Summit School
Observations
  • 8 legs (4 per side)
  • large abdomen (stripey pattern)
  • no antennae
  • no wings
  • 2 pedipalps
Putative identification Arthropoda Chelicerata Arachnida Araneae

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes
  • Use SeeGreen DNA dye in 1.5% agarose gel
  • Submerge Gel in TBE buffer
  • Use of Mini PCR Electrophoresis System
  • 5μl of DNA ladder
  • 2μl loading dye (SeeGreen) + 8μl DNA in each lane
  • Positive control (arthropod positive for Wolbachia)
  • Negative control (water)

Results
Wolbachia presence Unknown
Confidence level High
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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