Ant

April 1, 2025

Sample information
Picture
Location
Collection date 09/17/2024
Captive / Cultivated? Wild-caught
Group St. Paul Academy and Summit School
Observations

I collected this ant outside on a sidewalk. It was around 5 pm but relatively warm out. There were a lot of other ants but this was the biggest one.
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

The quality of the bases was relatively high overall and the appearance of the ant matched its highest DNA match after the nucleotide BLAST.
Wolbachia 16S sequence Download AB1
Arthropod COI sequence Download FASTA    Download AB1
Summary The Hymenoptera was found to be postive for Wolbachia.
Report Inappropriate Post