Beetle

Sample information

Picture
Location
Collection date 10/13/2020
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations
Putative identification Hexapoda Insecta Coleoptera

Methods

Extraction kit Edwards Buffer
DNA extraction location Abdomen
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Visible pellet

 

Results

Wolbachia presence No
Confidence level Medium
Explanation of confidence level

Arthropod gel: Arthropod controls didn’t work. Water shouldn’t have a band. 

Wolbachia gel: Wolbachia DNA didn’t work. Primer dimers found

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Coleoptera was found to be negative for Wolbachia.
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