Sample information
Picture	Photos by: Sachleen D.
Location
Collection date	09/03/2024
Captive / Cultivated?	Wild-caught
Group	Benedictine University
Observations
Putative identification	Arthropoda Insecta Diptera Sciomyzidae Dictya Dictya floridensis
Methods
Extraction kit	DNeasy (Qiagen) blood and tissue kit
DNA extraction location	Abdomen
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	TAE
DNA stain	SYBR Safe
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	Medium
Explanation of confidence level	medium band 100% ID Match in BLAST
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Dictya floridensis was found to be negative for Wolbachia.