Sample information |
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| Picture |
|
|---|---|
| Location | |
| Collection date | 09/19/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Gettysburg College |
| Observations | Rural area near wetland |
| Putative identification | Arthropoda Hexapoda Insecta Diptera Culicidae |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | Lane 11 |
Results |
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| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | Positive control is positive and negative control is negative |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
AAGATATTGGAACATTATACTTCATCTTTGGAGTTTGATCAGGAATAGTTGGTACATCATTAAGAATTTTAATTCGTGCTGAATTAAGTCAACCAGGAATATTTATTGGAAATGACCAAATTTATAATGTAATTGTTACAGCTCATGCTTTTATTATAATTTTCTTTATAGTTATACCTATTATAATTGGAGGATTCGGAAATTGATTAGTTCCTCTTATACTAGGAGCACCAGATATAGCTTTCCCTCGAATAAATAATATAAGTTTCTGAATATTACCTCCTTCATTAACTCTACTACTTTCAAGTAGTATGGTAGAAAATGGATCAGGAACAGGATGAACTGTTTATCCCCCTCTTTCATCTGGAACTGCTCATGCTGGAGCTTCTGTTGATTTAACTATTTTTTCTCTTCATTTAGCAGGAGTATCATCAATTTTAGGAGCTGTAAATTTTATTACTACTGTTATTAATATACGATCTGCAGGAATTACTTTAGATCGATTACCATTATTTGTTTGATCTGTTGTTATTACAGCTGTTTTATTACTTTTATCTCTTCCTGTATTAGCCGGAGCTATTACAATATTGTTAACTGACCGAAATCTAAATACATCCTTCTTTGACCCAATTGGTGGGGGAGACCCAATTCTATACCAACATTTATTCTGATTTT
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Culicidae was found to be negative for Wolbachia. |
Water Boatman