Sample information |
|
| Picture |
|
|---|---|
| Location | No map location data available. |
| Collection date | 10/11/2020 |
| Captive / Cultivated? | Wild-caught |
| Group | William Fremd High School |
| Observations | This insect was collected in a field with tall grass. |
| Putative identification | Arthropoda Hexapoda Insecta Orthoptera |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | This extraction was performed in a professional lab. |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | Medium |
| Explanation of confidence level | There were some issues with the amount of DNA. The band is smeared in the area for Wolbachia however. The controls worked well. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Orthoptera was found to be postive for Wolbachia. |
Water Boatman