Cricket

March 28, 2021

Sample information
Picture
Location No map location data available.
Collection date 10/11/2020
Captive / Cultivated? Wild-caught
Group William Fremd High School
Observations

This insect was collected in a field with tall grass.
Putative identification Arthropoda Hexapoda Insecta Orthoptera

Methods
Extraction kit Edwards Buffer
DNA extraction location Abdomen
Single or Duplex PCR
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

This extraction was performed in a professional lab.

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

There were some issues with the amount of DNA. The band is smeared in the area for Wolbachia however. The controls worked well.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Orthoptera was found to be postive for Wolbachia.
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