Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 11/14/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Hawaii Community College |
| Observations | (Leafhopper?) was in my backyard in the morning around 7:40 when I collected it. |
| Putative identification | Arthropoda Hexapoda Insecta Hemiptera |
Methods |
|
| Extraction kit | pcr |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Edvotek Gel Electrophoresis |
| Buffer | TBE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | There was absolutely no possible band showing any signs of Wolbachia. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Hemiptera was found to be negative for Wolbachia. |
Water Boatman