Unknown species

December 9, 2024

Sample information
Picture
Photos by: Aiyanna D
Location
Collection date 11/14/2024
Captive / Cultivated? Wild-caught
Group Hawaii Community College
Observations

(Leafhopper?) was in my backyard in the morning around 7:40 when I collected it.
Putative identification Arthropoda Insecta Hemiptera

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Edvotek Gel Electrophoresis
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes

Gel lanes (Top)

  1. DNA ladder
  2. Sample 1 CO1
  3. Sample 1 16S

 

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

There was absolutely no possible band showing any signs of Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hemiptera was found to be negative for Wolbachia.
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