Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 09/04/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Benedictine University |
| Observations | Collected by Fall 2024 students |
| Putative identification | Arthropoda Hexapoda Insecta Orthoptera Tettigoniidae Conocephalus Conocephalus brevipennis |
Methods |
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| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
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| Protocol notes | |
Results |
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| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | 99.59% Identity (BLAST Match) |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
Download AB1
TATTTAAATTTCGATCAGTTAATAATATAGTAATAGCTCCAGCTAATACTGGTAAAGATAATAATAAAAGTAGTGCTGTA ATAGCTACGGCCCATACAAATAAGGGAGTTTGATCCAGGGATATTCCTGGAGCTCGTATATTAATTGTTGTAGTAATAAA ATTTACAGCTCCTAAAATAGAGGAAATACCTGCTAAATGTAATGAGAAGATCGCTAAATCGACTGAGGCTCCTGCATGGG CAATATTAGCAGAAAGTGGAGGGTAAACTGTTCAACCAGTGCCTGCCCCGTTTTCGACAAGGCTTCTGGCTAGAAGCAAG GTCAAAGATGGGGGTAAAAGTCAAAAACTCATATTGTTTATTCGAGGGAAAGCTATGTCTGGGGCTCCCAGTATTAAAGG AACTAATCAATTCCCGAATCCTCCGATTATAATTGGTATAACTATAAAAAAAATTATAACAAAGGCATGTGCTGTTACAA TAACGTTATAA
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Conocephalus brevipennis was found to be negative for Wolbachia. |
Water Boatman