Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 09/03/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Benedictine University |
| Observations | Collected by Fall 2024 student |
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera Formicidae Formica Formica incerta |
Methods |
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| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
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| Protocol notes | |
Results |
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| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | My Gel bands were thick, showing high amounts of Arthropod DNA as well as Wolbachia DNA. When I put my DNA sequence into BLAST, I also got |
| Wolbachia 16S sequence | Download FASTA
ATTCCCATGGCGTGACGGGCAGTGTGTACAAGACCCGAGAACGTATTCACCGTGGCGTGNTGATCCACGATTACTAGCGA TTCCAACTTCATGCACTCGAGTTGCAGAGTACAATCCGAACTGAGATGGCTTTTAAGGGATTAGCTTAGCCTCGCGACTT TGCAGCCCATTGTAGCCACCATTGTAGCACGTGTGTAGCCCACTCCATAAGGGCCATGATGACTTGACATCATCCCCACC TTCCTCCAGTTTA
BLAST at The Wolbachia Project BLAST at NCBI
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| Arthropod COI sequence | Download FASTA
TTAAGTTTCGATCAGTTAATAATATTGTAATAGCTCNAGCTAAAACGGGAAGGGATAATAAAAGTAAAATTGCAGTGATT AAGATAGATCATACAAGTAGGGGAATTTTATCAATAGAAAAATTTTTATGATGTATATTANNGATTGTTGAAATAAAATT AATAGCTCCTAAAATAGATGATATACCGGCAATATGAAGAGAAAAAATTGTTAAGTCTACAGAAGGCCCATTATGAAAAA TATTTGAAGCTAAAGGANGGTANATAGTTCACCCAGTTCCAGTACCATCATTAATAAAGTTTCTTAAAAGTAATAGAGTG ANGNANGGAGGTAAAAGTCAAAATCTTATATTATTTATACGAGGATAAGCTATATCTGGNGATCCTAATATTAAAGGAAT TAGAAAATTTCCNAATCCACCAATTATAAATGGTATTACTATAAAAAAGA
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Formica incerta was found to be postive for Wolbachia. |
Water Boatman