Brachinus nigriventris (1)

February 14, 2025

Sample information
Picture
Location
Collection date 02/14/2025
Captive / Cultivated? Captive / Cultivated
Group John Overton High School
Observations

The exact origin of this insect is unknown due to the fact that it was purchased online. More research to be done.
Putative identification Arthropoda Hexapoda Insecta Coleoptera Carabidae Brachinus Brachinus nigriventris

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR
Gel electrophoresis system MiniPCR
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Shards of abdomen were present in the sample, leading to smears in the gel.

Results
Wolbachia presence Unknown
Confidence level Low
Explanation of confidence level

No bands were present.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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