A. punctipennis (Woodland Malaria Mosquito)

April 3, 2025

Sample information
Picture
Photos by: Charlotte FB.
Location
Collection date 02/15/2025
Captive / Cultivated? Wild-caught
Group Berkshire Community College
Observations

Flying through living room in winter.
Putative identification Arthropoda Hexapoda Insecta Diptera Culicidae Anopheles Anopheles punctipennis

Methods
Extraction kit Monarch DNA extraction (NEB)
DNA extraction location body without wings
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Edvotek Gel Electrophoresis
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

TAQ polymerase: NE Bio Labs ONETAQ Hot Start Quick-Load 2x MM w/ Standard Buffer (product #M0488S).

DNA Ladder: New England Biolabs 1 kb Plus DNA Ladder for Safe Stains (product #N0559S)

Annealing temperature at 49C.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The controls worked as expected, and the lines are very defined at the fragment levels.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Anopheles punctipennis was found to be negative for Wolbachia.
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