Sample information
Picture	Photos by: Ainsley M., Kabir G., Erin T.
Location
Collection date	02/28/2025
Captive / Cultivated?	Wild-caught
Group	Singapore American School
Observations	It’s a big ant, around 1-2mm in length
Putative identification	Arthropoda Hexapoda Insecta Hymenoptera Formicidae
Methods
Extraction kit	DNeasy (Qiagen) blood and tissue kit
DNA extraction location	Whole arthropod
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	MiniOne
Buffer	TBE
DNA stain	Other
Gel images
Protocol notes
Results
Wolbachia presence	Yes
Confidence level	Medium
Explanation of confidence level	We are confident in our results because the controls worked.
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Formicidae was found to be postive for Wolbachia.