Ant – AinsleyErinKabir

February 27, 2025

Sample information
Picture
Photos by: Ainsley M., Kabir G., Erin T.
Location
Collection date 02/28/2025
Captive / Cultivated? Wild-caught
Group Singapore American School
Observations

It’s a big ant, around 1-2mm in length
Putative identification Arthropoda Hexapoda Insecta Hymenoptera Formicidae

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain Other
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

We are confident in our results because the controls worked.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Formicidae was found to be postive for Wolbachia.
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