Sample information |
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Picture |
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Location | |
Collection date | 02/28/2025 |
Captive / Cultivated? | Wild-caught |
Group | Singapore American School |
Observations | Roach with missing legs |
Putative identification | Arthropoda Hexapoda Insecta Blattodea Blattidae Periplaneta Periplaneta americana |
Methods |
|
Extraction kit | DNeasy (Qiagen) |
DNA extraction location | Abdomen |
Single or Duplex PCR | Single Reaction |
Gel electrophoresis system | MiniOne |
Buffer | lysis |
DNA stain | Other |
Gel images |
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Protocol notes | |
Results |
|
Wolbachia presence | No |
Confidence level | High |
Explanation of confidence level | The roach did not test positive for Wolbachia, this is because the PCR meant to amplify the DNA did not show in the electrophoresis stage. |
Wolbachia 16S sequence | |
Arthropod COI sequence |
|
Summary | The Periplaneta americana was found to be negative for Wolbachia. |