American Roach Singapore American School

March 3, 2025

Sample information
Picture
Photos by: Huanzhi Z., Amber H., Winnie X., Jesse B.
Location
Collection date 02/28/2025
Captive / Cultivated? Wild-caught
Group Singapore American School
Observations

Roach with missing legs
Putative identification Arthropoda Insecta Blattodea Blattidae Periplaneta Periplaneta americana

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer lysis
DNA stain Other
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The roach did not test positive for Wolbachia, this is because the PCR meant to amplify the DNA did not show in the electrophoresis stage.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Periplaneta americana was found to be negative for Wolbachia.
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