Yellow Mealworm (Tenebrio Molitor)

April 2, 2025

Sample information
Picture
Photos by: Emily F.
Location
Collection date 02/19/2025
Captive / Cultivated? Captive / Cultivated
Group Berkshire Community College
Observations

Was still in larvae form not a beetle
Putative identification Arthropoda Hexapoda Insecta Coleoptera Tenebrionidae Tenebrio Tenebrio molitor

Methods
Extraction kit Monarch DNA extraction (NEB)
DNA extraction location Partial abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Edvotek Gel Electrophoresis
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes
  • March 5th PCR
  • taq polymerase use was New England Biolabs
  • annealing temperatures for arthropod reaction was 49 celsius, for denaturing was 94 celcuis, and extension 68 celcius
  • March 26th PCR
  • taq polymerase used New England Biolobs
  • Duplex PCR we used both Wolbachia and Arthropod primers
  • DNA ladder used New England Biolabs
  • annealing temperatures for arthropod reaction was 49 celsius

Results
Wolbachia presence Unknown
Confidence level Medium
Explanation of confidence level

It is unknown if my arthropod will have Wolbachia, Our controls looked good our problem is that our arthropod DNA doesn’t seem to be there, and next time we would need to add more DNA to get a more conclusive result.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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