Sample information
Picture	Photos by: Aminah Mir
Location
Collection date	01/12/2025
Captive / Cultivated?	Wild-caught
Group	Penn State Lehigh Valley
Observations	In the landscape
Putative identification	Arthropoda Crustacea
Methods
Extraction kit	DNeasy (Qiagen) blood and tissue kit
DNA extraction location	Abdomen
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system Bio-Rad
Buffer	TAE
DNA stain	SYBR Safe
Gel images
Protocol notes
Results
Wolbachia presence	Yes
Confidence level	High
Explanation of confidence level	i’m
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Crustacea was found to be postive for Wolbachia.