Lady Beetle: Group 4

March 20, 2025

Sample information
Picture
Photos by: Dalani P., Angel F., Jessica G.C., Natali R.
Location
Collection date 02/20/2025
Captive / Cultivated? Wild-caught
Group Penn State Lehigh Valley
Observations

Found in window sill
Putative identification Arthropoda Hexapoda Insecta Coleoptera Coccinellidae

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

1- Water(-)

2- DNA Ladder

3- Arthropod 1

4- Arthropod 2

5- Arthropod(+)

6- Arthropod(-)

7- DNA(+)

8- Water(-)

9- BLANK

10- DNA Ladder

11- Wolbachia 1

12- Wolbachia 2

13- Wolbachia(+)

14- Wolbachia(-)

15-DNA(+)

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

Novice
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Coccinellidae was found to be negative for Wolbachia.
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